Alloimmunized Fetal Blood Group Genotyping (Allo-FBGG)

If you have certain antibodies in your blood, Alloimmunized Fetal Blood Group Genotyping (Allo‑FBGG) can check if your baby has the matching blood group marker (called an antigen) that your antibodies might react to.

This helps your care team understand if your pregnancy could be at risk for hemolytic disease of the fetus and newborn (HDFN) and whether you may need extra monitoring.

This test is publicly funded for Ontario residents.

This test is publicly funded for Ontario residents who are pregnant and have one or more of these red blood cell antibodies:

  • anti-D
  • anti-C
  • anti-c
  • anti-E
  • anti-K (Kell) 

Who should not have this test?

Allo-FBGG is NOT recommended if you:

  • Have antibodies other than anti-D, anti-C, anti-c, anti-E or anti-K (Kell)
  • Started the pregnancy with more than one baby and one was lost
  • Have anti-D antibodies that have been confirmed to be passive (for anti-D only)
  • Have a known RHD variant or weak D blood type (for anti-D only)

The timing of when the tests is done depends on the antibody:

  • Anti-D, anti-C, anti-c and anti-E are tested from 16 weeks of pregnancy
  • Anti-K (Kell) is tested from 20 weeks of pregnancy

This test uses a sample of your blood. During pregnancy, small pieces of genetic material from the placenta circulate in your blood. The lab looks at this material to determine if your baby has the blood group antigen that your antibody reacts to.

Your blood can be drawn at a community lab and sent to Mount Sinai Hospital for testing. Use our map to find a lab near you. 

Results are usually ready in about 10 business days and are sent to your health-care provider.

Antigen-Positive Antigen-Negative
Your baby has the blood group antigen tested for and is at risk of hemolytic disease of the fetus and newborn (HDFN). Your provider will discuss extra monitoring for your pregnancy. This may include blood tests to check the amount of antibody in your blood, ultrasounds to measure blood flow to the baby, and a referral to a maternal-fetal medicine (MFM) specialist. Your baby does not have the blood group antigen tested for and is not at risk of HDFN. No change to your prenatal care is needed. Your health-care provider will continue to provide routine prenatal care.

What if the test cannot give a result?

Sometimes the test cannot give a result. Another blood sample may be requested to repeat the test, or it may be recommended to treat your pregnancy as if your baby has the antigen and provide closer monitoring.

Learn more about Fetal Blood Type Results & Next Steps

After delivery, a small sample of umbilical cord blood will be collected to confirm your baby’s antigen status. These results may be used to guide newborn care and for laboratory quality checks.

Watch the Alloimmunized Fetal Blood Group Genotyping Information Video

Download the Fetal Blood Group Genotyping for Alloimmunized Pregnancies Information Guide for Providers

Download the Fetal Blood Group Genotyping for Alloimmunized Pregnancies Information Guide for Pregnant Individuals

Contact Us

Prenatal Screening Ontario 
CHEO Research Institute 
Centre for Practice-Changing Research Building 
401 Smyth Road 
Ottawa, ON K1H 8L1

pso@bornontario.ca

Information line
Phone: 613-737-2281
Toll-Free: 1-833-351-6490

Monday to Friday, 9:00 AM to 3:00 PM EST

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Virtual educational sessions on prenatal screening and nuchal translucency quality assurance are available upon request for health‑care providers.